Fig. 3: Schematic model of development of marker used to detect 1.2-kbp insertion mutation across exons 1 and 2 of BrMAM-3.

a Insertion identified in accession Z16. Amplified region used to detect the insertion mutation encompassed part of exon 1, intron 1, and part of exon 2. F forward primer, R reverse primer. b Amplified fragments of BrMAM-3 in the accessions L143 and Z16