Fig. 6: Functional complementation analysis of BrMAM-3 gene in the Arabidopsis natural mutant Ler-0.

The BrMAM-3 gene is overexpressed in Ler-0, and the glucosinolate content and profile (in µnmol g−1 dry weight) was determined in 8-week-old rosette leaves. The individual graphs show the accumulation of a total aliphatic glucosinolates; b total indol glucosinolate. Three independent mutant-complemented lines of BrMAM-3 gene were analyzed, and average foliar glucosinolates from 30 individual plants are represented along with their standard errors. Asterisks indicate significant differences in glucosinolate content compared with Ler-0 (P < 0.05, one-way ANOVA analysis with Duncan post hoc test). Dashed lines indicate the average amount of total aliphatic/indol glucosinolates