Fig. 3: Construction of the mutant eTM of lncRNA23468. | Horticulture Research

Fig. 3: Construction of the mutant eTM of lncRNA23468.

From: Tomato lncRNA23468 functions as a competing endogenous RNA to modulate NBS-LRR genes by decoying miR482b in the tomato-Phytophthora infestans interaction

Fig. 3

a Predicted base-pairing interactions between miR482b and the eTM of lncRNA23468 with the designed mutations. b Strategy for introducing mutations by PCR. c PCR products from the different stages of the mutant eTM of lncRNA23468. M, DL2000 DNA Marker; 1, 2, and 3, products from the first, second and third rounds of PCR, respectively. d The sequencing results of the eTM regions of lncRNA23468 (top) and its mutated sequence (bottom). The red box represents the eTM region. Blue triangles represent the six-point mutation. e Sequence alignment of lncRNA23468 and mlncRNA23468 performed with ClustalX. The red box represents the eTM region

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