Fig. 5: Silencing of lncRNA23468 and NBS-LRR gene decreases tomato resistance to P. infestans. | Horticulture Research

Fig. 5: Silencing of lncRNA23468 and NBS-LRR gene decreases tomato resistance to P. infestans.

From: Tomato lncRNA23468 functions as a competing endogenous RNA to modulate NBS-LRR genes by decoying miR482b in the tomato-Phytophthora infestans interaction

Fig. 5

a Relative expression levels of lncRNA23468, miR482b and the target genes of miR482b in the TRV2 and lncRNA23468-VIGS tomato plants. b The silencing efficiency of NBS-LRR in NBS-LRR-VIGS tomato plants. c Phenotypes of the detached leaves from TRV2, lncRNA23468-VIGS and NBS-LRR-VIGS tomato plants at 5 dpi. Scale bars = 0.5 cm. Top: disease symptoms; Bottom: trypan blue staining for detection of dead cells. d The diameter of the lesion of the detached leaves. e Leaf phenotypes at 5 dpi after whole-plant inoculation with P. infestans. Scale bars = 0.5 cm. f Disease index of VIGS-tomato plants at 5 dpi. g Transcript accumulation of the P. infestans actin gene in these inoculated plants at 5 dpi. All data are the means ± SE of three independent experiments. Different letters among the groups indicate a significant difference at the P = 0.05 level

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