Fig. 2: Kinetics analysis of 2 fructokinase isoform proteins from E. coli expressing MdFRKs. | Horticulture Research

Fig. 2: Kinetics analysis of 2 fructokinase isoform proteins from E. coli expressing MdFRKs.

From: Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves

Fig. 2

a Schematic gene structure of pSUMO-M/MdFRK1 and pSUMO-M/MdFRK2 constructs. b SDS-PAGE and western blot results. For MdFRK1 (left): Lanes M1, M2, protein markers; 1, MdFRK1 (2.00 μg); 2, BSA (2.00 μg); 3, FRK1 (His antibody). For MdFRK2 (right): Lanes M1, M2, protein markers; 1, MdFRK2 (2.00 μg); 2, BSA (2.00 μg); 3, FRK2 (His antibody). c Effect of fructose concentration on MdFRK1 (left) and MdFRK2 (right) activity in extracts prepared from E. coli. Inset shows the level at which fructokinase activity inhibition begins

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