Fig. 7: MdMYB1 bound to the MdGSTF6 promoter.
From: MdGSTF6, activated by MdMYB1, plays an essential role in anthocyanin accumulation in apple
a Y1H assay showing the interaction between MdMYB1 and the MdGSTF6 promoter. The empty pGADT7 vector was used as a control. b Chromatin immunoprecipitation (ChIP) assay showing binding of MdMYB1 to the G1 region of the MdGSTF6 promoter in vivo. Apple ‘Orin’ calli overexpressing HA protein was used as a control. c Electrophoretic mobility shift assay (EMSA) result showing the interaction between MdMYB1 and labeled probes in the MdGSTF6 promoter. Red letters indicate MYB-binding site (MBS) and mutated MBS (MdGSTF6m). Lane 2 contains labeled DNA probes and MdMYB1 protein without a competitor. Increasing amounts (10×, 25×, and 50×) of unlabeled normal DNA probes (MdGSTF6 in lanes 3, 4, and 5 of each blot) or unlabeled mutated DNA probes (MdGSTF6m in lanes 7, 8, and 9 of each blot) were added as cold competitors. Lane 6 contains labeled mutated DNA probes and the MdMYB1 protein
