Fig. 4: Treatment and tissue dependent patterns of differentially expressed genes (DEGs).

a Heatmaps showing clustered expression patterns of DEGs by treatment in the hypanthium and ovary at 18 days after treatment. DEGs were identified by comparing across GA₃-treated, NAA-treated, hand pollinated, and negative control (ANOVA-like), with a fold difference ≥ 2 and a Bonferroni correction value ≤ 0.05. Each treatment contains at least three biological replicates, which cluster by treatment based on similarity in expression pattern. Gray = hand-pollinated, Fuchsia = GA₃-treated, Yellow = NAA-treated, Black = negative control. Normalized expression values range from < 0.4 (bright blue) to > 1.6 (bright red). Total number of DEGs identified for 18 DAT: hypanthium = 8946, ovary = 12,271. b Venn diagrams showing relationships between sets of DEGs. Hand pollinated, GA₃-treated and NAA-treated are compared with negative control. DEGs are determined with a fold difference ≥ 2 and a Bonferroni correction value ≤ 0.05. HP hand pollinated, GA₃ GA₃-treated, NAA NAA-treated, NC negative control