Fig. 5: Effect of incubation time on transient gene manipulation. | Horticulture Research

Fig. 5: Effect of incubation time on transient gene manipulation.

From: Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits

Fig. 5

Detached LG fruits were infected with Agrobacterium carrying the eGFP gene driven by the 35CaMV promoter, and the fruits were incubated for different times at 25 °C and 100% humidity. a eGFP images on different days after Agrobacterium infection. b eGFP image of a representative fruit, showing the altered pattern a relatively long time after Agrobacterium infection. C. qRT-PCR analysis of eGFP gene expression. FaACTIN was used as a normalized control gene. Values are means ± SD of six fruits. Statistically significant differences among samples were determined by Tukey’s test, and significant differences at the P < 0.05 level are indicated by different letters. D. Immunoblot analysis of eGFP protein; each lane represents a mixed sample of three fruits from different plants

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