Fig. 6: Effect of fruit developmental stage on transient gene manipulation. | Horticulture Research

Fig. 6: Effect of fruit developmental stage on transient gene manipulation.

From: Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits

Fig. 6

Detached fruits of different stages were infected by injection of Agrobacterium carrying the vector containing both the eGFP and GUS reporter genes driven by the 35CaMV promoter. Five days after infection, eGFP protein was imaged, and GUS activity was stained. a Fruits at different developmental stages: SG small green fruit, MG mid-sized green fruit, LG large green fruit, W white fruit. b eGFP images. c GUS staining. d Immunoblot analysis of eGFP protein; each lane represents a mixed sample of three fruits from different plants

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