Table 3 Summary of conditional optimization

From: Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits

Factor

Conditional optimization

Notes

•Vector type

•Means of infection

•Temperature

•Time of infection

•Developmental stage

•Variety

•TGM inconsistency

•VIGS vector is not recommended

•Detached fruits, full injection

•20~25 ℃

•4–6 days after initial infection

•LG to W stage

Fragaria ananassa “Benihoppe”

•Adoption of mixed fruit sample for measuring fruit-ripening-related parameters; adoption of PDP for gene function research

•Infection until whole fruit fully infiltrated

•The most critical factor