Table 2 Overview of miRNA sequencing reads
Sample | Total reads | N% > 10% | Low quality | 5′-Adapter contamine | 3′-Adapter null or insert null | With poly A/T/G/C | Clean reads | |
|---|---|---|---|---|---|---|---|---|
Drought-sensitive plants | Control 1 | 33,928,632 (100%) | 371 (0%) | 81,721 (0.24%) | 33,287 (0.10%) | 1,377,648 (4.1%) | 53,145 (0.16%) | 32,382,460 (95%) |
Control 2 | 31,905,071 (100%) | 360 (0 %) | 84,190 (0.26%) | 32,884 (0.10%) | 555,157 (1.7%) | 70,210 (0.22%) | 31,162,270 (98%) | |
Control 3 | 33,330,848 (100%) | 349 (0%) | 77,489 (0.23%) | 32,009 (0.10%) | 653,356 (2.0%) | 58,660 (0.18%) | 32,508,985 (98%) | |
Drought 1 | 32,537,917 (100%) | 347 (0%) | 82,237 (0.25%) | 27,717 (0.09%) | 875,074 (2.7%) | 37,609 (0.12%) | 31,514,933 (97%) | |
Drought 2 | 24,779,273 (100%) | 252 (0%) | 66,295 (0.27%) | 25,252 (0.10%) | 709,788 (2.9%) | 64,004 (0.26%) | 23,913,682 (97%) | |
Drought 3 | 30,900,174 (100%) | 328 (0%) | 75,907 (0.25%) | 34,188 (0.11%) | 775,123 (2.5%) | 67,303 (0.22%) | 29,947,325 (97%) | |
Drought-tolerant plants | Control 1 | 31,489,922 (100%) | 443 (0%) | 86,581 (0.27%) | 28,714 (0.090%) | 1,352,490 (4.3%) | 39,785 (0.13%) | 29,981,909 (95%) |
Control 2 | 29,888,571 (100%) | 430 (0%) | 78,512 (0.26%) | 32,389 (0.11%) | 855,209 (2.9%) | 58,814 (0.20%) | 28,863,217 (97%) | |
Control 3 | 26,866,676 (100%) | 435 (0%) | 73,980 (0.28%) | 24,177 (0.090%) | 448,026 (1.7%) | 62,656 (0.23%) | 26,257,402 (98%) | |
Drought 1 | 29,371,046 (100%) | 444 (0%) | 73,889 (0.25%) | 31,098 (0.11%) | 504,062 (1.7%) | 51,860 (0.18%) | 28,709,693 (98%) | |
Drought 2 | 30,422,600 (100%) | 445 (0%) | 85,569 (0.28%) | 26,499 (0.090%) | 978,522 (3.2%) | 45,285 (0.15%) | 29,286,280 (96%) | |
Drought 3 | 33,456,352 (100%) | 479 (0%) | 82,842 (0.25%) | 28,253 (0.080%) | 1,266,189 (3.8%) | 29,138 (0.090%) | 32,049,451 (96%) | |
