Fig. 3: Transcriptional activation activity analyses of CmMYB15 and the DNA-binding assay of AC elements with CmMYB15.

a The transcriptional activity analysis of CmMYB15 via a yeast assay system, in which pCL1 is a positive control and pGBKT7 is a negative control. b Yeast one-hybrid (Y1H) assay of CmMYB15 with the three kinds of AC elements: AC-I, AC-II, and AC-III, as well as their mutated form mAC