Fig. 1: Molecular properties of SlARF6A. | Horticulture Research

Fig. 1: Molecular properties of SlARF6A.

From: Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato

Fig. 1

a Expression pattern of SlARF6A revealed by the expression of the GUS reporter gene driven by the SlARF6A promoter. Gus staining was conducted using leaf, shoot, bud, flower, and fruit tissues from transgenic plants at 2, 4, 8, 30, and 45 days post anthesis (DPA). The bar is 1 mm. b Subcellular localization analysis of SlARF6A protein. The SlARF6A-GFP fusion protein and GFP-positive and GFP-negative controls (PCXDG-GFP) were transiently expressed in tobacco (Nicotiana benthamiana) leaves. Images were taken in a dark field for green fluorescence, while the outline of the cells and the merged image were recorded in a bright field. The bar is 15 μm. c Transcriptional activation activity of SlARF6A protein. The pGBKT7-SlARF6A fusion vector, negative control (Empty pGBKT7 vector) and positive control were transformed into Y2H gold yeast cells. The yeast cells were cultivated on medium without tryptophan (SD-Trp) or without tryptophan, histidine, and adenine (SD-Trp/His/Ade)

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