Fig. 7: SlARF6A binds to the promoters of SlGLK1, CAB and RbcS genes and promotes the transcription of these genes. | Horticulture Research

Fig. 7: SlARF6A binds to the promoters of SlGLK1, CAB and RbcS genes and promotes the transcription of these genes.

From: Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato

Fig. 7

a Diagrams of the reporter and effector constructs used in the dual-luciferase reporter assay. b In vivo interactions of SlARF6A with the promoters obtained from transient assays in tobacco leaves. The ratio of LUC/REN of the empty vector plus promoter was used as a calibrator (set as 1). Each value represents the mean ± SD of six biological replicates. c ChIP-qPCR assay for direct binding of SlARF6A to the promoters. Values are the percentage of DNA fragments that coimmunoprecipitated with anti-FLAG antibodies or nonspecific antibodies (anti-IgG) relative to the input DNA. The data represent the mean ± SD of four biological replicates. d, e, f, g EMSA showing the binding of SlARF6A to the promoters of CAB1, CAB2, RbcS, and SlGLK1, respectively. Biotin-labeled DNA probes from native promoters or mutants were incubated with GST-SlARF6A protein, and the DNA-protein complexes were separated on 6% native polyacrylamide gels

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