Fig. 8: SlARF6A binds to the SAMS1 promoter and inhibits SAMS1 transcription. | Horticulture Research

Fig. 8: SlARF6A binds to the SAMS1 promoter and inhibits SAMS1 transcription.

From: Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato

Fig. 8

a Diagrams of the reporter and effector constructs used in the dual-luciferase reporter assay. b In vivo interactions of SlARF6A with the promoter obtained from transient assays in tobacco leaves. The ratio of LUC/REN of the empty vector plus promoter was used as a calibrator (set as 1). Each value represents the mean ± SD of six biological replicates. c ChIP-PCR assay for direct binding of SlARF6A to the SAMS1 promoter. Values are the percentage of DNA fragments that coimmunoprecipitated with anti-FLAG antibodies or nonspecific antibodies (anti-IgG) relative to the input DNA. The data represent the mean ± SD of four biological replicates. d EMSA showing the binding of SlARF6A to the SAMS1 promoter

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