Fig. 3: Expression pattern of Md-miRln20 and Target Gene among various apple germplasms with different resistance to GLS.

a The incidence index of apple germplasms. ‘Hanfu’, ‘GL-3’, ‘M26’, ‘Greensleeves’, ‘JM2’, ‘B9’, ‘Meigumi-1’, and ‘Malus hupehnensis Rhed’ were tissue-cultured plantlets, and others were grown in the field. b, c Expression level of Md-miRln20 (b) and Target Gene (c) in leaves before and after inoculation. d The incidence index of 10 susceptible and 10 resistant F1 hybrids of ‘Golden Delicious’ × ‘Nagafu 2’. e, f The expression level of Md-miRln20 (e) and Target Gene (f) in leaves of hybrids before and after inoculation