Fig. 4: Validation of a predicted target of Md-miRln20. | Horticulture Research

Fig. 4: Validation of a predicted target of Md-miRln20.

From: A novel miRNA negatively regulates resistance to Glomerella leaf spot by suppressing expression of an NBS gene in apple

Fig. 4

a Secondary structure of the miRNA precursor and the location of Md-miRln20 (red). b Md-miRln20 targets the 5′ UTR of Md-TN1-GLS. Blue boxes indicate exons. Lines between bases indicate Watson–Crick base pairs, dots indicate wobble base pairs, and the asterisk indicates a mismatched pair. c Conserved domains of Md-TN1-GLS predicted by Pfam. d The 5′ UTR of Md-TN1-GLS containing the complementarity site was fused to the GUS reporter gene under control of the CaMV 35S promoter (35S::TN15′UTR-GUS). (1) and (2) Representative images of leaves expressing various constructs. EV, empty vector of pFGC5941; MIRLN20, overexpression of the precursor sequence of Md-miRln20 under control of the 35S CaMV promoter. Bar = 0.4 cm. e Quantification of relative GUS expression levels in transformed leaves was performed in three biological replicates. Statistical significance was determined by Student’s t-test: *P < 0.05

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