Fig. 4: Verification of homozygous deletion mutant lines. | Horticulture Research

Fig. 4: Verification of homozygous deletion mutant lines.

From: Targeted deletion of floral development genes in Arabidopsis with CRISPR/Cas9 using the RNA endoribonuclease Csy4 processing system

Fig. 4

a Primer sets were used to amplify the editing regions between two DSBs in AP1, SVP, and TFL1 separately. b–d Agarose gel electrophoresis of the fragments of targeted genes. The absence of all three fragments indicated plants with homozygous deletions. WT wild type. e Efficiencies of targeted deletion with different mutation types in T1 plants

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