Table 1 Summary of targeted deletions with the CRISPR/Cas9 system
# sgRNAs | Sequence 5′–3′ | PAM 5′–3′ | GC content (%) | Target loci | Deletion mutants/# of Hyg+T1 plants |
|---|---|---|---|---|---|
sgRNA1 | GGGGTAGGGTTCAATTGAAG | AGG | 50 | AP1 exon 1 | 8/23 |
sgRNA2 | AGATACTTGAACGCTATGAG | AGG | 40 | AP1 exon 2 | |
sgRNA3 | GACATCGGCGTCGCAGAGAA | CGG | 60 | SVP exon 1 | 5/17 |
sgRNA4 | ACTGCAAGTTATGCCTCTCT | AGG | 45 | SVP exon 2 | |
sgRNA5 | CTTCTGTTTCCTCCAAGCCT | AGG | 50 | TFL1 exon 1 | 10/30 |
sgRNA6 | ATGATAGACCCAGATGTTCC | AGG | 45 | TFL1 exon 2 |
