Table 2 Targeted mutagenesis of a single target site with CRISPR/Cas9 using multiplexed sgRNAs
Target gene | Indel at sgRNA site #1 only | Indel at sgRNA site #2 only | Indel at both sgRNA sites | Inversion between sgRNA sites | Deletion between sgRNA sites | Total mutant plants |
---|---|---|---|---|---|---|
AP1 | 3 | 2 | 5 | 1 | 13 | 24 |
SVP | 0 | 1 | 1 | 0 | 9 | 11 |
TFL1 | 2 | 1 | 15 | 2 | 15 | 35 |