Table 2 Targeted mutagenesis of a single target site with CRISPR/Cas9 using multiplexed sgRNAs

From: Targeted deletion of floral development genes in Arabidopsis with CRISPR/Cas9 using the RNA endoribonuclease Csy4 processing system

Target gene

Indel at sgRNA site #1 only

Indel at sgRNA site #2 only

Indel at both sgRNA sites

Inversion between sgRNA sites

Deletion between sgRNA sites

Total mutant plants

AP1

3

2

5

1

13

24

SVP

0

1

1

0

9

11

TFL1

2

1

15

2

15

35