Fig. 5: Characterization of transcription activation of ACS1 by MYB108A.
From: Melatonin enhances salt tolerance by promoting MYB108A-mediated ethylene biosynthesis in grapevines
a Sequences containing MBS or mMBS elements for yeast one-hybrid assay and underlined sequence for EMSA. b Yeast one-hybrid assay using MBS and mMBS as bait. (1) pAbAi-MBS/pGAD-MYB108A; (2) pAbAi-MBS/pGAD7; (3) pAbAi-mMBS/pGAD-MYB108A; (4) pAbAi-mMBS/pGAD7. c Interaction of the MYB108A protein with labeled DNA probes for cis-elements of the ACS1 promoter in EMSA. d, f Histochemical analysis of the transactivation activity of MYB108A via the binding MBS element. ‘Crimson seedless’ calluses d and tobacco leaves f were agro-infiltrated with different vector constructs: D1, WT calluses; D2, MBS-35S mini-GUS; D3, mMBS-35S mini-GUS and 35S::MYB108A; D4, MBS-35S mini-GUS and 35S::MYB108A; F1, WT leaf; F2, PACS1::ACS1-GUS; F3, PACS1::ACS1-GUS and 35S::MYB108A. e GUS activities of grape calluses infiltrated by Agrobacterium containing D1-D4 constructs. g ACS1 expression and GUS activities of tobacco leaves infiltrated by Agrobacterium containing F1-F3 constructs. Each image is representative of three biological replicates in b, c, d, and f. In e and g, values are presented as the means ± SD of 3 replicates
