Fig. 5: Overexpression of SlOFP20 affects BR-related genes.
From: Overexpression of SlOFP20 affects floral organ and pollen development
a The expression levels of SlOFP20 at different time points under 10 μΜ EBR treatment. b–h Comparison of BR-related gene expression between WT and overexpression lines. Each value represents the mean ± SE of three replicates. * indicates a significant difference (P < 0.05) between the wild-type and transgenic lines. i Yeast two-hybrid assay for the SlOFP20 & SlKNOX1, SlOFP20& SlGRAS41 and SlKNOX1 & SlGRAS41 proteins. QDO, SD medium without Trp, Leu, His, and Ade; QDO/X, QDO medium with X-a-Gal. 1. pGBKT7-53 & pGADT7-T (positive control); 2. pGBKT7-Lam & pGADT7-T (negative control); 3. pGBKT7-SlOFP20 & pGADT7-SlKNOX1; 4. pGBKT7-SlOFP20 & pGADT7-SlGRAS41; 5. pGBKT7-SlKNOX1& pGADT7-SlGRAS41; Empty bait vector, empty prey vector, and autoactivation assay with no growth of yeast
