Fig. 7: RhMYB108 directly binds to the promoter of RhNAC053, RhNAC092 and RhSAG113. | Horticulture Research

Fig. 7: RhMYB108 directly binds to the promoter of RhNAC053, RhNAC092 and RhSAG113.

From: RhMYB108, an R2R3-MYB transcription factor, is involved in ethylene- and JA-induced petal senescence in rose plants

Fig. 7

a qRT-PCR analysis of SAG expression in RhMYB108-silenced petals. b Interaction of RhMYB108 protein with the SAG promoter regions, as revealed using yeast one-hybrid assays. Interactions were determined based on yeast cell growth and were confirmed by the color indication of X-β-gal on SD/-Trp/-Ura medium plates. c, d Images of firefly luciferase fluorescence signals and relative reporter activity (LUC/REN) in N. benthamiana leaves. RhMYB108 protein was separately coexpressed with pNAC053::LUC, pNAC092::LUC and pSAG113::LUC in tobacco leaves. After 48 h of Agrobacterium tumefaciens infiltration, live LUC images and relative LUC activity (LUC/REN) in tobacco leaves were assayed. The error bars represent the means of six biological replicates, and the asterisks indicate statistically significant differences according to Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).

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