Fig. 3: PuMYB21 and PuMYB54 bound to the PuPLDβ1 promoter. | Horticulture Research

Fig. 3: PuMYB21 and PuMYB54 bound to the PuPLDβ1 promoter.

From: PuMYB21/PuMYB54 coordinate to activate PuPLDβ1 transcription during peel browning of cold-stored “Nanguo” pears

Fig. 3

a The growth status of transformed yeasts on two types of media. Normal yeast growth on defective medium containing the antibiotic aureobasidin A (−Leu + AbA800) indicates the binding of PuMYB21 and PuMYB54 to the promoter of PuPLDβ1. b Electrophoretic mobility shift assay (EMSA) results showing the interaction between PuMYB21/54 and the labeled probes in the PuPLDβ1 promoter. Bold letters indicate the MYB-binding site (MBS), which were biotin-labeled (hot probe). Red letters represent mutated MBS (mutant cold probe). The cold probe represents an unlabeled sequence. The symbols + or − represent presence or absence, respectively, and 200× indicates the amount of cold probe

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