Fig. 8: Interaction between PuMYB21 and PuMYB54. | Horticulture Research

Fig. 8: Interaction between PuMYB21 and PuMYB54.

From: PuMYB21/PuMYB54 coordinate to activate PuPLDβ1 transcription during peel browning of cold-stored “Nanguo” pears

Fig. 8

a The self-activating activity of PuMYB54. I pGBKT7 empty vector. II pGBKT7-PuMYB54 in Y2H. III pGBKT7-PuMYB54C in Y2H. IV pGBKT7-PuMYB54N in Y2H. b The N terminus of PuMYB54 interacted with PuMYB21. Y2H Yeast strain containing the bait plasmid PuMYB54N was transformed into PuMYB21. QDO is medium without Leu, Trp, His, or Ade. X-α-gal is QDO with X-α-gal. SV40 and P53 served as the positive control, and the others were negative controls. c A pull-down assay verified the interaction between PuMYB21 and PuMYB54. Escherichia coli-expressing GST or PuMYB54-GST fusion proteins were incubated with cobalt chelate affinity resin containing the immobilized histidine-tagged MdBT2 protein. The protein mixtures were purified using a HIS purification kit. d Bimolecular fluorescence complementation assays showing the interaction between PuMYB21 and PuMYB54 in the nuclei of onion epidermal cells

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