Fig. 2: Analysis of PCD occurrence and ROS levels in MdMIPS1-RNAi apple lines. | Horticulture Research

Fig. 2: Analysis of PCD occurrence and ROS levels in MdMIPS1-RNAi apple lines.

From: Myo-inositol mediates reactive oxygen species-induced programmed cell death via salicylic acid-dependent and ethylene-dependent pathways in apple

Fig. 2

Trypan blue a, NBT, and DAB staining d and H2O2 levels in the leaves e of GL-3 and MdMIPS1-RNAi apple lines under MI-depleted MS conditions. Representative TEM images of cell structure b, relative autophagic activity c, and superoxide dismutase (SOD) activity f, peroxidase (POD) activity g and anti-O2− activity h in aerial parts of GL-3 and Ri-1 under greenhouse conditions. The data are the means ± SDs (n ≥ 14 for c, more than 14 cells were used to quantify the structures; n = 3 for e, three biological replicates; n = 4 for f–h, four biological replicates). In comparison with GL-3, ***P < 0.001, **P < 0.01, and *P < 0.05. cw cell wall; c chloroplast; v vacuole; and s starch. The arrows indicate autophagic bodies. Bar = 2 μm for b.

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