Fig. 4: Analysis of ET production in MdMIPS1-RNAi apple lines. | Horticulture Research

Fig. 4: Analysis of ET production in MdMIPS1-RNAi apple lines.

From: Myo-inositol mediates reactive oxygen species-induced programmed cell death via salicylic acid-dependent and ethylene-dependent pathways in apple

Fig. 4

a Transcript levels of ET biosynthetic genes in GL-3 and Ri-2 leaves under MI-depleted MS conditions. b ET production in GL-3 and transgenic apple lines under tissue culture conditions. c–e Effects of CoCl2 application on GL-3 and Ri-2 under MS tissue culture conditions. f and g Effects of 1-MCP application on GL-3 and Ri-1 under greenhouse conditions. The data are the means ± SDs (n = 3 for a, three biological replicates; n = 12 for b and d, 12 biological replicates). e and g derived from two independent experiments with similar results, and one representative data point is shown. In comparison to GL-3, ***P < 0.001. The values not represented by the same letter are significantly different (P < 0.05). FW fresh weight. e Levels 1–4 indicate injury severity: (1) ≤1 leaf with necrosis on every plant; (2) 2–3 leaves; (3) 4–5 leaves; and (4) ≥6 leaves.

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