Fig. 5: P. viticola development and H2O2 production at inoculation sites in leaf discs of WT and VvPR4b knockout lines as revealed by staining with aniline blue and DAB. | Horticulture Research

Fig. 5: P. viticola development and H2O2 production at inoculation sites in leaf discs of WT and VvPR4b knockout lines as revealed by staining with aniline blue and DAB.

From: CRISPR/Cas9-mediated VvPR4b editing decreases downy mildew resistance in grapevine (Vitis vinifera L.)

Fig. 5

a–d and e–f represent 24 hpi and 72 hpi, respectively. a1–h1 show infected stomata stained with aniline blue under epifluorescence UV-microscopy. a2–h2 show the tissues stained with DAB under bright-field illumination in the field of vision corresponding to (a1–h1). Arrowhead, H2O2 accumulation; gt germ tube, st stomata, sv substomatal vesicle, ph primary hyphae, hy hyphae, sph sporangiophores, sp sporangia

Back to article page