Fig. 4: Expression of mixed mature amiRNAs for diverse treatments.

Expression levels of mixed mature miRNAs in Nicotiana benthamiana (a) and Solanum lycopersicum (b) after they were sprayed with pBI121–LDH (CK), pDNA1–LDH (amiRNA-AV1/AV2-1), pDNA2–LDH (amiRNA-AV1-2), pDNA3–LDH (amiRNA-AV1-3), and pDNA(1 + 2 + 3)-LDH (mixed with three pDNA–LDH), as determined by qRT-PCR assays. The bars for each column represent standard deviations. Values (means ± SEs) are the averages of three independent experiments, and significant differences (P ≤ 0.05) among treatments in each group are indicated by different letters