Fig. 2: Marker dependent abundance of terpenes and LOD scores for chromosome 4.

a Abundance of terpene in the VOM14 F₂ biparental mapping population sorted after marker genotyping. Three hundred and twenty plants were analysed by headspace GC-MS and the terpene content was sorted by the results of the marker analysis (KASP 4-31,228,189 CAPS 4-31,218,832). Association consistencies between the abundance of β-myrcene, limonene, sabinene, α-terpinene, γ-terpinene, terpinen-4-ol, α-thujene and 4-carene, and genotype in leaf and root tissue of carrot are presented in box and whiskers (min to max, show all points) with a fold change of the abundance in log10 scale. The data were statistically analysed with Tukey’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, not significant (ns)). b LOD score of terpenes associated with chromosome 4 based on markers flanking the terpene synthase gene cluster. Significance threshold (TH) was set using 1.000 permutations. c Validation of the KASP 4-31,228,189 marker. KASP-based genotyping of 85 carrot accessions. Association consistency between the abundance of β-myrcene, limonene, sabinene, α-terpinene, γ-terpinene, terpinen-4-ol, α-thujene and 4-carene and genotype in leaf and root tissue of carrot. All measured points were statistically analysed by Tukey’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, not significant (ns)) and presented in box and whisker plots. The abundance is shown with a fold change on a log10 scale. The horizontal x-axis (a, c) indicates genotypic groups a, b and h. The y-axis (a, c) indicates an abundance of terpenes in investigated plants and is on a log10 scale. The plants in group a carry the homozygous maternal allele (a), whereas the plants in group b harbour the homozygous paternal allele (b). Individuals in group h are the heterozygous plants