Fig. 3: Characterization of a genomic region associated with sabinene production on chromosome 4. | Horticulture Research

Fig. 3: Characterization of a genomic region associated with sabinene production on chromosome 4.

From: The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

Fig. 3

a Schematic representation of the TPS-gene cluster. Structural analysis of this region was performed based on reannotation of carrot genome assembly by GeMoMa (version 1.6.3.beta) using 12 reference genomes22,28. Receptor-like kinase (RLK) and galacturonosyltransferases (GAUT). b Amino acid sequence alignment of candidate terpene synthases from the yellow and cola mutant. Background in grey shows different residues in all five candidate terpene synthases. Differences between DcTPS04 and DcTPS54 sequences are marked in red. N-terminus of the catalytic domain (RR) and the DDIYD-conserved motif are marked in bold. The N-terminal plastid transit peptide predicted by Target P2.0 is marked with a horizontal line. The phylogram in the lower-left corner shows similarity of the catalytic domain of the 5 terpene synthases based on the translation of the nucleotide sequences from the yellow and the cola mutant. TPS-b marks the as TPS-b clade. A phylogenetic tree of all carrots TPS was previously published in ref. 23

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