Fig. 4: Expression profile of terpene synthases from gene cluster on chromosome 4. | Horticulture Research

Fig. 4: Expression profile of terpene synthases from gene cluster on chromosome 4.

From: The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

Fig. 4

The levels of RNA transcripts for DcTPS04, DcTPS26, DcTPS27, DcTPS54 and DcTPS55 genes were analysed in roots (mix of cortex, xylem and phloem tissues) and leaves (mix of young leaf and petiole tissues). Each sample was pooled from three plants. Each experiment was performed in biological and technical triplicates. Relative expression for the five TPS gene in yellow and cola mutants was calculated using the Pfaffl method with the reference genes DcTub, DcHSP, DcPP2A and presented with a fold change in a log10 scale. Relative to DcTub expression levels are presented in plots with standard deviation. Data were statistically analysed by unpaired t-Test (*P < 0.05, **P < 0.01, ****P < 0.0001). Samples with non-detectable CTs for three biological replicates are marked as nd

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