Fig. 3: Expression analysis of CmSI. | Horticulture Research

Fig. 3: Expression analysis of CmSI.

From: Melon short internode (CmSi) encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling

Fig. 3

a qRT-PCR analysis of CmSI expression in different organs of melon. b CmSI expression in stem internodes at different developmental stages. The melon gene ACTIN was used as the internal control. Error bars indicate standard deviations of three biological replicates. R root, S stem, L leaf, MF male flower, O ovary on the day of flowering. c–f mRNA in situ hybridization of CmSI in stems at 1 week after transplanting. CmSI is highly expressed in the vascular bundle of TopMark stems (c, d) and is decreased in stems of the M406 mutant (e, f). g Negative controls hybridized in stem with the sense probe. Bars = 100 µm in c–g

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