Fig. 5: Binding of MdSND1 to SNBE motifs in the promoters of the MdMYB46 and MdMYB83 genes. | Horticulture Research

Fig. 5: Binding of MdSND1 to SNBE motifs in the promoters of the MdMYB46 and MdMYB83 genes.

From: Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

Fig. 5

A MdSND1-binding sites in the MdMYB46 and MdMYB83 gene promoters. B SNBE site sequences in the MdMYB46, MdMYB83A, and MdMYB83B promoters. The SNBE sequence is marked in red. C EMSA showing the competitive binding of MdSND1 to SNBE fragments with unlabeled DNA probe (200-fold). D Vector structure of the effector and reporter used for tobacco transient expression assays. E Relative luciferase activity in the transient expression assays. F ChIP-qPCR-based analysis showing MdSND1 binding to the promoters of MdMYB46 and MdMYB83. The error bars indicate the standard deviations (SDs) of three biological replicates, *P < 0.05 (according to t-tests)

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