Fig. 7: MdSND1 can directly bind to the SNBE motif in the promoter of stress signaling genes. | Horticulture Research

Fig. 7: MdSND1 can directly bind to the SNBE motif in the promoter of stress signaling genes.

From: Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

Fig. 7

A Positions of SNBEs, DRE/CRTs, and ABREs in the promoters of MdAREB1A, MdAREB1B, MdDREB2A, MdRD22, and MdRD29A. B ChIP-qPCR assay showing MdSND1 binding to the promoters of MdAREB1A, MdAREB1B, MdDREB2A, MdRD22, and MdRD29A. The error bars indicate the standard deviations (SDs) of three biological replicates, *P < 0.05 (according to t-tests). C SNBE sequence in the promoter of stress-responsive signaling genes. The SNBE sequence is marked in red. D EMSA results showing the competing binding of MdSND1 to SNBE fragments with unlabeled DNA probe (200-fold). E Vector structure of the effector and reporter used for the tobacco transient expression assays. F Relative luciferase activity in the transient expression assays. The error bars indicate the standard deviations (SDs) of three biological replicates. The letters indicate the significance level (P < 0.05, according to Duncan’s multiple range test)

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