Fig. 2: Effects of SlNAP1 overexpression on plant growth and fruit phenotype.

a Detection of the SlNAP1-HA fusion protein in two independent homozygous SlNAP1-overexpressing lines (OE-SlNAP1-1 and -2) by western blot analysis using an anti-HA (Pierce) monoclonal antibody and the large subunit of Rubisco (RBC; as a loading control) in this experiment. b, c, d Plant phenotype in the early growth stage and growth indexes. The images were taken at 40 days (c) after sowing. Bar = 5 cm. Plant height and width were determined every 5 days after 25 days of sowing. e Plant phenotype in the advanced reproductive stage. Bar = 10 cm. f, g Fresh weight per fruit (g) and ripened fruit phenotype. Bar = 1 cm. h, j Fruit number per plant and yield (kg/m2). i Dry matter contents of tomato plant roots, stems, leaves, and fruits. The results are presented as the averages ± SDs of five plants, which were grown in a greenhouse. The different letters indicate significant differences between treatments (P < 0.05, Tukey’s test)