Fig. 2: MdGRF11 interacts with MdBT2 proteins. | Horticulture Research

Fig. 2: MdGRF11 interacts with MdBT2 proteins.

From: The apple 14-3-3 protein MdGRF11 interacts with the BTB protein MdBT2 to regulate nitrate deficiency-induced anthocyanin accumulation

Fig. 2

a Yeast two-hybrid assay to examine the interactions of MdGRF11 with MdBT1, MdBT2, MdBT3.1, MdBT3.2, and MdBT4. Yeast cells were grown on SD/-Trp-Leu (-T/-L) and SD/-Trp-Leu-His-Ade (-T/-L/-H/-A) media. X-α-gal was used to further confirm the positive interactions. b Y2H assay to determine the domain through which MdBT2 interacts with MdGRF11. c In vitro pull-down assay indicating the interaction of MdBT2 and MdGRF11. His-MdGRF11 proteins and GST-MdBT2 or GST fusion proteins were incubated together and then purified with a GST column. Immunoprecipitated proteins were detected with anti-HIS and anti-GST antibodies. IB immunoblotted. d Interaction between MdBT2 and MdGRF11 detected by a BiFC assay in transiently transformed N. benthamiana leaf epidermal cells. DAPI was used as a marker for the nucleus. YFP fluorescence was observed via confocal microscopy. Scale bars, 10 μm

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