Fig. 1: Screening and analyzing the candidate WRKY genes from transcriptome data of red-skinned pear ‘Starkrimson’ and its green mutant by bioinformatics.

a WRKY gene transcript abundance in ‘Starkrimson’ (Pyrus communis L.) and its green mutant at different developmental stages. R1, R2, and R3 indicate the 40, 55, and 85 DAFB samples of the red-skinned pear of ‘Starkrimson’; G1, G2, and G3 indicate the 40, 55, and 85 DAFB samples of the green mutant of ‘Starkrimson’. b WRKY genes of different species are analyzed in the phylogenetic tree. The analysis of the alignments of the C-terminal WRKY domain was performed using the neighbor-joining method by the MEGA7 Program. c Multiple sequence alignment of AtWRKY4 (At1g13960.1), PhPh3 (AMR43368), FvWRKY44 (XM_004302784.1), NtWRKY2 (AB063576), PtWRKY (XM_002326290.1), PyWRKY26 (Pbr013092.1), PyWRKY31 (Pbr000122.1) and AtWRKY6 (At3g58710). Identical, conserved and similar residues are shown in black, red and blue. WRKY-DNA domains are shown in squares with β2, β3, and β4. d Chromosomal localization of the WRKY genes in pear