Fig. 4: The functional analysis of PyWRKY31/PyWRKY26 cotransformed with its partners, which resulted in anthocyanin biosynthesis, as shown by transient expression assays in strawberry receptacles. | Horticulture Research

Fig. 4: The functional analysis of PyWRKY31/PyWRKY26 cotransformed with its partners, which resulted in anthocyanin biosynthesis, as shown by transient expression assays in strawberry receptacles.

From: PyWRKY26 and PybHLH3 cotargeted the PyMYB114 promoter to regulate anthocyanin biosynthesis and transport in red-skinned pears

Fig. 4

a The appearance of the strawberry receptacles 5 d after infiltration: a, pSAK277; b, PyMYB10; c, PyMYB114; d, PybHLH3; e, PyWRKY31; f, PyWRKY26; g, PyMYB10 + PybHLH3; h, PyMYB114 + PybHLH3; i, PyMYB114 + PyWRKY31; j, PyMYB114 + PyWRKY26; k, PyMYB10 + PyMYB114 + PybHLH3; l, PyMYB114 + PybHLH3 + PyWRKY31; m, PyMYB114 + PybHLH3 + PyWRKY26; n, PyMYB10 + PyMYB114 + PybHLH3 + PyWRKY31; o, PyMYB10 + PyMYB114 + PybHLH3 + PyWRKY26. b Differences in color are shown with the values of L*, a* and b*. Significant differences are indicated at the level of P < 0.05 with lowercase letters, and highly significant differences are shown at the level of P < 0.01 with uppercase letters. Error bars are based on six biological replicates. c Determination of the total anthocyanin contents. Error bars are based on three biological replicates. d–i RT-qPCR analysis of the expression levels of the genes FvDFR, FvANS FvUFGT, FvGST, FvABC transporter and FvAVP. Significant differences are indicated at the level of P < 0.05 with lowercase letters. Highly significant differences are indicated with uppercase letters at P < 0.01. Error bars show the SEs of the means (n = 3)

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