Fig. 5: The transactivate activity of TFs PyMYB10, PyMYB114, PybHLH3, and PyWRKYs for anthocyanin biosynthesis and transport related genes was verified by a dual-luciferase reporter assay.

The dual-luciferase reporter assay verified that PyMYB10, PyMYB114, PybHLH3 and PyWRKY26/PyWRKY31 cotransformation induces the activity of the PyDFR (a), PyANS (b), PyUFGT (c), PyGST (d), PyABC transporter (e) and PyAVP1/2 (f, g) promoters. The LUC/REN value was used to express promoter activity. Error bars represent the SEs for three replicate reactions