Fig. 6
From: CPPU may induce gibberellin-independent parthenocarpy associated with PbRR9 in ‘Dangshansu’ pear
Binding and stimulation of the PbYUCCA4 and PbNCED6 promoters by PbRR9. a Yeast one-hybrid assay showing the interaction of PbRR9 with the PbYUCCA4 and PbNCED6 promoters. b, c Validation of the activation effect of PbRR9 on the PbYUCCA4 (b) and PbNCED6 promoters (c) based on a dual luciferase assay. Relative promoter activity is represented by the ratio of the structural gene luciferase (LUC) to 35 S Renilla (REN). An empty vector was used as the reference. d–f The relative expression levels of PbRR9 (d), PbYUCCA4 (e), and PbNCED6 (f) according to a transient assay. Significant differences among treatments were determined by Student’s t-test: *P < 0.05; **P < 0.01. Error bars represent the standard deviation of the mean (SD; n = 3)
