Fig. 3: Transgenic evaluation of the impact of various indels on the AMAT promoter activity.

a Four original AMAT promoters (2.8–3.3 kb upstream and 133 bp downstream of ATG) from ‘Concord’, ‘Barry’, ‘Alba’ and ‘Caco’ and four mutated AMAT promoters, Alba-A, Caco-A, Caco-B, and Caco-C, were individually cloned into a GUS reporter binary vector. These vectors covered the indel variants of 426 bp (purple triangle), 26 bp (green triangle) and 42 bp (red triangle) in different genetic background. b Relative AMAT promoter activities measured by qRT-PCR expression of the reporter GUS gene in transgenic Arabidopsis seeds. Mature seeds from 9 to 20 independent T2 lines for each construct were pooled for total RNA extraction. Two to three batches of qRT-PCR were performed for each sample with three replicates each time. Error bar is the range of fold change based on standard deviation of ΔCt value