Fig. 1: MdSE reduces the transcript and protein levels of MdMYB88 and MdMYB124 by interacting with them. | Horticulture Research

Fig. 1: MdSE reduces the transcript and protein levels of MdMYB88 and MdMYB124 by interacting with them.

From: Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis

Fig. 1

a MdSE interacts with MdMYB88 and MdMYB124 in tobacco leaves by BiFC analysis. Bars = 20μm. b MdSE interacts with MdMYB88 in tobacco by Co-IP analysis. MdMYB88-HA was coinfiltrated with MYC-MdSE in tobacco leaves. Proteins were extracted and immunoprecipitated with an anti-HA antibody. The immunocomplex was then detected with western blot using anti-MYC or anti-HA. c, d Expression level of MdMYB88 or MdMYB124 in GL-3, MdSE RNAi, and MdSE OE plants under control or drought conditions. Data are means ± SD (n = 3). Student’s t test was performed, and statistically significant differences are indicated by *P < 0.05, **P < 0.01, or ***P < 0.001. e Protein level of MdMYB88 or MdMYB124 in GL-3, MdSE RNAi, and MdSE OE plants under control or drought conditions

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