Fig. 3: DNA methylation landscape of CYP450 family and other TSB-related genes in P. grandiflorus genome. | Horticulture Research

Fig. 3: DNA methylation landscape of CYP450 family and other TSB-related genes in P. grandiflorus genome.

From: Whole-genome, transcriptome, and methylome analyses provide insights into the evolution of platycoside biosynthesis in Platycodon grandiflorus, a medicinal plant

Fig. 3

a Methylation status of the CYP450 family genes in the CG, CHG, and CHH contexts. Based on the distribution of methylation levels of all protein-coding genes (including gene bodies and ~2-kb upstream and downstream regions) in the control sample (CT 12 h_AllGenes; black line), the methylation levels of CYP450 genes were measured in the control treatment (CT 12 h; red) and at three time points in the MJ treatment (MJ 12 h [blue], MJ 24 h [green], and MJ 48 h [orange]). b Density of differentially methylated cytosines (DMCs) in all three contexts in CYP450 and other TSB-related genes between the control and MJ treatments (CT vs. MJ 12 h, CT vs. MJ 24 h, and CT vs. MJ 48 h). c Abundance of CG-DMCs in the top 30% CYP450 and TSB-related genes in the MJ treatment. d Relative abundance of hypermethylated and hypomethylated cytosines (hyper-DMCs and hypo-DMCs, respectively) in the CG context of CYP716s, CYP72s, CYP76s, CYP71s, GGPSs, and bAS in the MJ treatments. The ratio was transformed to the log2 scale. e Relative abundance of hyper-DMCs and hypo-DMCs in the CG context within ~2-kb upstream regions and coding regions of the primary CYP450 and TSB-related genes. f Identification of genes showing inverse correlation between CG methylation levels and gene expression in the MJ treatment. Asterisk (*) indicates a gene with similar DMC distribution in the ~2-kb upstream region and coding region

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