Fig. 6: HPLC-MS analysis of DcaCGT C-glucosyltransferase reactions using 2-hydroxynaringenin and phloretin as substrates.
From: Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
Cells on the left show chromatograms of the enzymatic activity assays using 2-hydroxynaringenin as a substrate a with inactivated protein; b with activated protein before acid treatment; and c with activated protein after acid treatment. d Standards for related compounds: 1, 2-hydroxynaringenin; 2, isovitexin; 3, vitexin; 4, vicenin-2. Cells on the right show chromatograms for the enzymatic activity assays using phloretin as a substrate e with inactivated protein; f incubated for 5 min; and g incubated for 90 min. h Standards for related compounds: 7, phloretin; 8, nothofagin. MS2 spectra for the proposed intermediate products: i 5, 6-C-glucosyl-2-hydroxynaringenin; j 6, 6,8-di-C-glucosyl-2-hydroxynaringenin. k MS2 spectra and l fragmentation of the proposed product 3’,5’-di-C-glucosylphloretin
