Fig. 3: Coimmunoprecipitation assay analysis of PGR5a and separation of thylakoid membrane protein complexes by blue native PAGE followed by immunoblotting. | Horticulture Research

Fig. 3: Coimmunoprecipitation assay analysis of PGR5a and separation of thylakoid membrane protein complexes by blue native PAGE followed by immunoblotting.

From: The key cyclic electron flow protein PGR5 associates with cytochrome b6f, and its function is partially influenced by the LHCII state transition

Fig. 3

a CsPGR5a-HA, CsPetC, CsLhcb3, and CsPsaH were coexpressed in N. benthamiana, and CoIP was performed by using an anti-HA antibody. Tobacco thylakoid membranes coexpressing empty vectors together with HA, CsPetC, CsLhcb3, and CsPsaH were used as negative controls, and purified thylakoid membranes (Input) were used as positive controls. Western blotting was performed by using anti-PsaH, anti-PetC, and anti-Lhcb3 antibodies. b Immunoblotting analysis of cucumber thylakoid membrane protein complexes by using anti-PGR5 and anti-PetC antibodies. c BN-PAGE of tobacco thylakoid membrane proteins with or without overexpression of CsPGR5a, anti-PGR5, anti-PetC, anti-Lhcb3, and anti-PsaH antibodies was used for immunoblotting

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