Fig. 5: Overexpression of CsPGR5a in common tobacco. | Horticulture Research

Fig. 5: Overexpression of CsPGR5a in common tobacco.

From: The key cyclic electron flow protein PGR5 associates with cytochrome b6f, and its function is partially influenced by the LHCII state transition

Fig. 5

a Western blot of the WT and different transgenic lines (OE-5 and OE-2) using anti-GFP and anti-PGR5 antibodies. b GFP signal of tobacco leaves in the WT and different transgenic lines. c–e Phenotypes of tobacco seedlings, leaves, capsules, and seeds of the WT and different transgenic lines. The images of the seeds were taken by an S3000 scanning electron microscope (Hitachi, Tokyo, Japan). f Tobacco PGR5a was knocked out in the OE-5 line, and the phenotypes of OE-5 pgr5#11 and OE-5 pgr5#11 recovered to a phenotype similar to that of the WT. g–i Leaf area, leaf weight per area, and Chl content per area of WT, OE-5, OE-5 pgr5#11, and OE-5 pgr5#11. j, k Two pmf components of WT, OE-5, OE-5 pgr5#11, and OE-5 pgr5#11. The different letters indicate significant differences between treatments (P < 0.05) according to Tukey’s test

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