Fig. 6: BN-PAGE and western blotting of tobacco thylakoid membrane proteins. | Horticulture Research

Fig. 6: BN-PAGE and western blotting of tobacco thylakoid membrane proteins.

From: The key cyclic electron flow protein PGR5 associates with cytochrome b6f, and its function is partially influenced by the LHCII state transition

Fig. 6

The pgr5 overexpression and deficient lines were developed in the background of common tobacco NC89. The PGR5a coding sequence was cloned into cucumber cDNA. To explain whether PGR5a in cucumber and tobacco has the same function, we knocked out PGR5a in tobacco in the CsPGR5 overexpression line. a BN-PAGE analysis of the thylakoid membrane complexes of WT, OE-5, OE-2, pgr5#101, pgr5#21, OE-5 pgr5#11, and OE-5 pgr5#11. b Western blotting analysis of complexes of WT, OE-5, OE-2, pgr5#101, pgr5#21, OE-5 pgr5#11, and OE-5 pgr5#11 by using anti-PGR5, anti-Lhcb3, anti-PetC, and anti-PsaH antibodies

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