Fig. 2: Y2H assay showing that PvAVH53 interacts with Vitis vinifera VvImpα/α4. | Horticulture Research

Fig. 2: Y2H assay showing that PvAVH53 interacts with Vitis vinifera VvImpα/α4.

From: Importin-αs are required for the nuclear localization and function of the Plasmopara viticola effector PvAVH53

Fig. 2

The short segment of PvAVH53 containing the NLS (124–134 amino acids) interacts with the two arm domains of VvImp α4, as evidenced by growth on DDO (minimal medium, double dropout: SDLeu/-Trp) and QDO/A/X (minimal medium, quadruple dropout: SD-Ade/-His/-Leu/-Trp) in the presence of Aba and Xα-Gal. Left panel: Schematic diagram of the full length and deletion constructs of VvImpα4 and PvAVH53. Right panel: Confirmation of the interaction by cotransformation of different plasmids

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