Fig. 3: PvAVH53 and VvImpαs localize to and interact in the Vitis vinifera cell nucleus.

a Subcellular localization of V. vinifera nuclear import factors VvImpα4/VvImpα, PvAVH53, and GFP as controls cotransformed with NLS-mCherry (as the nuclear localization marker) in V. vinifera protoplasts. b A BiFC assay confirmed that VvImpαs interacted with PvAVH53. Photos were taken after protoplasts were incubated for 20–24 h under weak lighting at 25 °C. Scale bar = 5–10 μm